Oligosaccharides Produced by Acetolysis of Blood Group Active (A + H)sulfated Glycoproteins from Hog Gastric Mucin
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چکیده
منابع مشابه
The use of ion exchange resins in the isolation of blood group A-specific substance from hog gastric mucin.
ion Ezchunye Resins-De-Acidite (The Permutit Company, New York), washed on a 40 mesh screen with distilled water, was treated several times with aqueous 4 per cent sodium carbonate and repeatedly washed with distilled water, by suspension and decantation, until the supernatant liquid was colorless and had a pH of 8.0 or less. The resin collected on a suction filter contained 70 to 80 per cent w...
متن کاملAn Examination of the Blood Group a Specific Substance from Hog Gastric Mucin and the Specific Polysaccharide of Bacterium Dysenteriae
a qualitative method for the analysis of the hydrolysis products of complex polysaccharides. Interest especially centres in the general class of mucopolysaccharides-polysaccharides which contain hexosamines and which frequently show a special capacity for forming combinations with proteinsthe reactions of which may be of considerable importance in the storage behaviour of foodstuffs. The specif...
متن کاملStructural study of the blood group A active glycolipids of hog gastric mucosa.
Two forms of blood group A active glycolipids (L and II) containing oligosaccharides of 7 sugar residues have been isolated from hog stomach mucosa lipid extract (SLOMIANY, A., AND HOROWITZ, M. I. (1973) J. Biol. Chem. 248, 62326238. Partial acid hydrolysis, infrared spectroscopy, periodate oxidation, permethylation, and sequential enzymatic degradation were used to elucidate the glycolipid str...
متن کاملStructure determination of oligosaccharides isolated from A+, H+ and A-H- hog-submaxillary-gland mucin glycoproteins, by 360-MHz 1H-NMR spectroscopy, permethylation analysis and mass spectrometry.
Alkaline borohydride reductive cleavage (beta-elimination) of hog submaxillary glycoproteins from three immunologically determined phenotypes, viz. A+, H+ and A-H-, resulted in the release of a series of neutral and acidic oligosaccharide-alditols. 360-MHz 1H-NMR spectroscopy in combination with methylation analysis and mass spectrometry were used for reinvestigation of the structures of these ...
متن کاملA spectrophotometric study of blood group A-specific substance isolated from hog gastric mucosa.
The isolation from hog gastric mucosa of homogeneous preparations of the polysaccharide-amino acid complex possessing the ability to inhibit the isoagglutination of human blood group A cells (1) has been hindered not only by dilliculties of manipulation and the presence in gastric mucosa of substances having properties similar to those of blood group A-specific substance, but also by the fact t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44108-2